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KMID : 0613820130230111360
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Journal of Life Science
2013 Volume.23 No. 11 p.1360 ~ p.1364
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Optimization of a Process for Extraction of Petasin from Petasites japonicus Leaves by Response Surface Methodology
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Lee Dong-Wan
Lee Se-Yeul
Chung Hun-Sik
Choi Young-Whan
Im Dong-Soon
Lee Young-Guen
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Abstract
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Petasin extracted from Petasites japonicus leaves has been well known to be effective in the treatment of allergic asthma. This study was carried out to optimize the extraction process of petasin from P. japonicus leaves by response surface methodology (RSM). The dried powder of P. japonicus leaves was extracted at ethanol concentrations ranging from 40% to 80%, extraction rpm ranging from 125 rpm to 225 rpm, and extraction time ranging from 1 to 3 hours. The effects of the extraction conditions on the dry yield and petasin content of the extracts were investigated using a second-order Box-Behnken design. The petasin content was significantly affected by ethanol concentration, extraction rpm, and extraction time, tending to increase more with increasing ethanol concentration. The optimum condition for petasin extraction from Petasites japonicus leaves was 79.92% in ethanol concentration, 178.10 rpm in extraction rpm, and 2.06 hours in extraction time, respectively.
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KEYWORD
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Box-Behnken design, extraction process, petasin, Petasites japonicus, response surface methodology
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